Endomicroscopy has become an increasingly popular topic in live imaging. With the explosion of endomicroscopy publications in the last decade, identifying the ones with high impact contribution can be difficult. In this summary from the Optiscan team, we highlight the technological advancement (Part 1) and milestone applications (Part2 – May 2021) of fluorescence in vivo endomicroscopy. Published in Microscopy Today by the Microscopy Society of America.

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